Conjugate Stablizers
Stabilize your conjugates for a more accurate assay.
ICT has formulated a group of seven unique stabilizing diluents designed to prolong the shelf-life of HRP-conjugated or AP-conjugated proteins and enhance their use in ELISA applications. These conjugate stabilizer diluents can be used to reconstitute lyophilized conjugates and to dilute concentrated conjugates into the useful range of the assay. HRP and AP-conjugated antibodies are often unstable and can lose a significant level of activity within a short time after rehydration and dilution unless properly stored. This loss of activity is based on two factors: denaturization of the HRP-IgG or AP-IgG protein complex; and destabilization of the catalytic region of the HRP or AP enzyme.
ICT has created 7 proprietary conjugate diluent formulations for use with sandwich and antigen-down ELISAs:
Monoclonal / Polyclonal Conjugate Stabilizer (CS1)
CS1 is a unique 5x formulation specifically designed for use in mouse monoclonal capture and polyclonal-HRP sandwich ELISA formats. This diluent contains mouse proteins which suppress nonspecific binding of the polyclonal-HRP conjugate to the mouse immunoglobulins adsorbed onto the plate. These protein additives also provide additional structural stability to the conjugate protein complex. Other additives contained in CS1 serve to extend the useful titer of the conjugate by stabilizing both the immunoglobulin and HRP components of the conjugate. Antimicrobial agents have also been added to retard bacterial growth. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.
Monoclonal / Polyclonal Conjugate Diluent & Stabilizer (CS1)
Antigen-Down Conjugate Stabilizer (CS2)
CS2 is a unique 5x formulation specifically designed for use with anti-IgG-HRP conjugates. This diluent contains proprietary non-mammalian protein additives which will not interfere with anti-IgG-HRP conjugates used in antigen-down and sandwich ELISA formats (it does not contain BSA). These additives also provide additional structural stability to the IgG-HRP conjugate protein complex. CS2 will also inhibit conjugate binding to nonspecific serum proteins bound to the plate, thus reducing conjugate bridging on the plate surface and lowering background noise. Antimicrobial agents have been added to retard bacterial growth. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.
Polyclonal / Polyclonal Conjugate Stabilizer (CS3)
CS3 is a unique 5x formulation specifically designed for use in polyclonal capture and polyclonal-HRP sandwich ELISA formats. Proprietary additives were specifically selected to provide optimal assay performance when using rabbit, goat, or rabbit and goat polyclonal antibodies in a sandwich ELISA format. CS3 contains a uniquely processed form of rabbit and goat IgG to suppress nonspecific binding of the polyclonal-HRP conjugates to the plate surface. To reduce nonspecific binding interactions, a detergent is also included. Antimicrobial agents have been added to retard bacterial growth. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.
Polyclonal / Polyclonal Conjugate Diluent & Stabilizer (CS3)
Monoclonal / Goat-Polyclonal Conjugate Stabilizer (CS4)
CS4 is a unique 5x formulation specifically designed for use in monoclonal capture and goat polyclonal-HRP sandwich ELISA formats. Additives were carefully selected to specifically address the stability and nonspecific binding issues associated with this type of ELISA format. Goat proteins are included to reduce any nonspecific interactions between the monoclonal capture and goat polyclonal-HRP antibodies. CS4 also contains mouse IgG to specifically suppress nonspecific bridging of the goat polyclonal-HRP conjugate with the mouse capture IgG on the plate surface. To further reduce nonspecific binding of the conjugate to the plate surface, a detergent is also included. Antimicrobial agents have been added to retard bacterial growth. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.
Monoclonal / Goat-Polyclonal Conjugate Diluent & Stabilizer (CS4)
Monoclonal / Rabbit-Polyclonal Conjugate Stabilizer (CS5)
CS5 is a unique 5x formulation specifically designed for use in monoclonal capture and rabbit polyclonal-HRP sandwich ELISA formats. Rabbit proteins are included to reduce any nonspecific interactions between the monoclonal capture and rabbit polyclonal-HRP antibodies. CS5 also contains mouse IgG to specifically suppress nonspecific bridging of the rabbit polyclonal-HRP conjugate with the mouse capture IgG on the plate surface. To further reduce nonspecific binding of the conjugate to the plate surface, a detergent is also included. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.
Monoclonal / Rabbit-Polyclonal Conjugate Diluent & Stabilizer (CS5)
Conjugate Stock Stabilizing Reagent (CS6)
CS6, supplied at 5x, contains a mammalian protein additive to stabilize conjugated proteins and salts to stabilize the catalytic site of the HRP porphyrin ring structure. When diluted to 1x, CS6 is an excellent HRP conjugate diluent and stabilizer base, to which additional components can be added to for use in your specific assay.
At 5x, CS6 is an excellent solution for stabilizing newly conjugated proteins. The liquid conjugate stock may be stabilized and stored for later use in the assay. Simply add 1 part of 5x CS6 (that has not been diluted to 1x) to 4 parts of the new conjugate solution. For example, add 5mL of 5x CS6 to 20mL of newly eluted antibody-HRP conjugate solution. If the conjugate was initially at 10 mg/mL, the stabilized conjugate will now be at 8 mg/mL in 25mL diluent (diluted by 20%).
CS6 contains antimicrobial agents to retard bacterial growth. When stored at 2°-8°C, unopened and protected from light, a shelf-life of 18 months can be expected.
Alkaline Phosphatase Enzyme Conjugate Stabilizer (CS7)
CS7 is useful in any assay that employs an antigen or antibody conjugated to alkaline phosphatase. AP conjugates are stable to a concentration of 2mcg/mL when diluted in this solution. CS7 is provided ready to use at 1x.
ICT’s proprietary conjugate stabilizer formulations preserve the integrity of the antibody binding regions while maintaining the enzymatic functionality of the HRP or AP enzyme.
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1-800-829-3194