5x Coating Buffers

Making a reliable ELISA starts with our Coating Buffer

The first step in making a reliable ELISA is proper coating of the antibody or antigen onto the plate. ICT has specifically formulated two buffers for coating antibodies and antigens onto polystyrene microtiter ELISA plates. Both coating buffers stabilize coated proteins by maintaining their tertiary three-dimensional structure, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal.

For antibody-sandwich ELISAs, use ICT's 5x Universal Antibody Plate Coating Buffer (CB1). CB1 stabilizes the structure of coated antibodies for proper reactivity and long-term stability on stored ELISA plates. It can also be used with antigen-down ELISAs.

For most antigen-down ELISAs, we recommend ICT's 5x Antigen Coating Buffer (CB2). This buffer is recommended for antigen-down ELISAs. CB2 stabilizes the structure of coated antigens for proper reactivity and long-term stability on stored ELISA plates.

Coating Buffer Frequently Asked Questions

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Block Buffers Conjugate Stabilizer Diluents Sample Diluents

Assay Diluents Wash Buffer Phosphate Buffered Saline

Substrates Stop Solutions Custom Lab Solutions

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