ELISA Solutions
Optimize your Assay's Performance from Start to Finish
An ELISA, or Enzyme-Linked ImmunoSorbent Assay, is a biochemical test used to detect an antibody or an antigen in a sample. ELISAs are used as diagnostic tools in medicine and plant pathology, as valuable investigative tools in research laboratories, and can play a critical role in quality control assessments.
ImmunoChemistry Technologies offers a comprehensive line of high-quality buffers, diluents, and stabilizers for the preparation and execution of ELISA tests. From start to finish, these reliable assay solutions help to control multiple variables and address complex issues including matrix effects, non-specific binding, and signal-to-noise ratio problems.
Build a Better Assay:
Plate Coating Buffers The first step in making a reliable ELISA is proper coating of the antibody or antigen onto the plate. ICT has specifically formulated 2 plate coating buffers for use with antibodies and antigens.
Block Buffers Once the antibody or protein antigens have been properly adsorbed onto the plate, the next critical step in creating a reliable assay is the blocking of the plate.
Conjugate Stabilizer Diluents ICT has formulated a group of seven unique stabilizing diluents designed to prolong the shelf-life of HRP-conjugated and AP-conjugated proteins and to enhance their utility in ELISA applications.
HRP-Conjugated Secondaries **NEW** AffiPure, Fc specific, goat anti-IgG isotype HRP conjugates for ELISA and Western Blotting
Conjugation-Ready HRP, Maleimide-Activated **NEW**
Assay Diluents ICT’s assay diluents are proprietary buffer formulations designed to equalize any differences between the sample matrix (serum, plasma, urine, cell culture fluid) and the diluent used to generate the standard curve.
Sample Diluents A sample diluent is a buffer used to dilute test samples so they read within the functional range of the assay. ICT has created 3 proprietary sample diluent formulations for use with antigen-down and sandwich ELISAs.
Wash Buffer WB1 is used to rinse microtiter plates during the coating process and between reagent addition steps of an ELISA. It is a universal ELISA wash buffer; it may be used with antibody-sandwich ELISAs and antigen-down ELISAs.
Phosphate Buffered Saline PB1 is a well-tested formulation of buffers and salts designed to effectively balance the pH without disrupting antibody-sandwich ELISAs and antigen-down ELISA binding reactions.
Substrates Substrates are used to generate the read-out signal of the ELISA assay.
Stop Solutions Stop Solutions are used to prevent further color development of the substrate in an ELISA assay.
Assay Development Kits ImmunoChemistry Technologies provides complete assay kits to facilitate the development of your own standard assay. Purchasing the complete Kit is more economical than buying the individual reagents.
Accessories
Essentials for ELISA development and manufacturing: 96-well plates, foil storage bags, adhesive plate sealing film, desiccant packs, and plate template notepads.
Expert Lab Services
Custom Lab Solutions Our business started this way! Consultation or services for each stage of assay development, from feasibility to plate coating.
ICT has specifically formulated several buffers, diluents, and stabilizers for immunoassay development and manufacturing.
Why Develop it Yourself When We Have the Solution?