Coming soon: Far-Red FLICA Caspase-1 Detection Probe

ICT is pleased to announce the upcoming release of a far-red fluorescence-emitting inhibitor probe for the in vitro detection of activated caspase-1 (ICE) enzyme.

FLICA™ 660 Caspase-1 Detection Reagent, 660-YVAD-FMK, allows for the rapid and reliable detection of caspase-1 in whole, living cells. Perform multiplexing studies with blue-, green-, or orange-labeled biomarkers via flow cytometry and fluorescence microscopy. The far-red 660-YVAD-FMK fluorescent reagent joins its green fluorescent analog for active caspase-1 detection, FLICA FAM-YVAD-FMK, in the ICT FLICA range of fluorescent probe assay kits.

 

Caspase-1 (ICE)

• Originally identified as Interleukin -1β Converting Enzyme or ICE
• Cleaves IL-1 beta and IL-18 into active forms
• Creation of the inflammasome during host responses to pathogens leads to the activation of caspase-1
• Active caspase-1 plays an antimicrobial role during infection, leading to pyroptosis
• Inflammation-related disease models have illustrated a role for caspase-1 in asthma, rheumatoid arthritis, multiple sclerosis and other disorders

 

 

  

Activated THP-1 cells show caspase-1 (ICE) activity with the green fluorescent FAM-FLICA Caspase-1 Detection Assay

 

 

 

 

 

 

 

FAM-FLICA™ Caspase-1 Assay

This in vitro assay employs a fluorescent green inhibitor probe to detect active caspase-1 enzymes in living cells or tissue samples.
View assay details and data: http://www.immunochemistry.com/products/green-flica-caspase-1-detection-assay-kit.html
 

 

• Direct, fast labeling of active caspase-1

• Flexible analysis with standard fluorescence detection methods

• Green fluorescence allows for multiplexing with blue, orange, and red fluorescent dyes

    

  

Citations for the green FAM-FLICA Caspase-1 Assay:

Our FLICA™ Caspase-1 Assay (catalog numbers 97 and 98, containing FAM-YVAD-FMK reagent) continues to be routinely referenced in customers' publications. Here are a few of the latest:

E. D. Murphey. Cecal Ligation and Puncture-Induced Impairment of Innate Immune Function Does Not Occur in the Absence of Caspase-1. J. Immunol. 2011; 187:905-910.

Mélanie Anne Hamon and Pascale Cossart. K+ efflux, is required for histone-H3 dephosphorylation by Listeria LLO and other pore forming toxins. Infect. Immun., Apr 2011; 10.1128/IAI.01243-10.

Norma Olivares-Zavaleta, Aaron Carmody, Ronald Messer, William M. Whitmire, and Harlan D. Caldwell. Chlamydia pneumoniae Inhibits Activated Human T Lymphocyte Proliferation by the Induction of Apoptotic and Pyroptotic Pathways. J. Immunol. 2011; 186:7120-7126.

 

  

 

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